Molecular Cloning and Characterization of Five Glutathione S-Transferase Genes and Promoters from Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae) and Their Response to Tannic Acid Stress.
Identifieur interne : 000263 ( Main/Exploration ); précédent : 000262; suivant : 000264Molecular Cloning and Characterization of Five Glutathione S-Transferase Genes and Promoters from Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae) and Their Response to Tannic Acid Stress.
Auteurs : Fang Tang [République populaire de Chine] ; Huizhen Tu [République populaire de Chine] ; Qingli Shang [République populaire de Chine] ; Xiwu Gao [République populaire de Chine] ; Pei Liang [République populaire de Chine]Source :
- Insects [ 2075-4450 ] ; 2020.
Abstract
Plants accumulate phenolic compounds such as tannic acid to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of glutathione S-transferases (GSTs). Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae) is an important foliar pest of poplar trees. GSTs play an important role in xenobiotic detoxification in M. troglodyta. Five GST genes were identified in M. troglodyta and were classified into five different cytosolic GST classes, delta, omega, sigma, theta, and zeta. Real-time fluorescent quantitative polymerase chain reaction (qPCR) was used to determine the mRNA expression of the five cloned GSTs in the midguts and fat bodies of M. troglodyta. The mRNA expression of the five GSTs was significantly induced when M. troglodyta was exposed to tannic acid. To further understand the tannic acid regulatory cascade, the 5'-flanking promoter sequences of the five MtGSTs were isolated by genome walking methods, and the promoters were very active and induced by tannic acid. In summary, the induction of GST mRNA expression was due to the response of five MtGST promoters to tannic acid. Therefore, MtGST promoters play an important role in the regulation of GST transcription.
DOI: 10.3390/insects11060339
PubMed: 32492871
PubMed Central: PMC7349759
Affiliations:
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Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Nanjing Forestry University, Nanjing 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author><author><name sortKey="Tu, Huizhen" sort="Tu, Huizhen" uniqKey="Tu H" first="Huizhen" last="Tu">Huizhen Tu</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Nanjing Forestry University, Nanjing 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Nanjing Forestry University, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author><author><name sortKey="Shang, Qingli" sort="Shang, Qingli" uniqKey="Shang Q" first="Qingli" last="Shang">Qingli Shang</name><affiliation wicri:level="1"><nlm:affiliation>School of Agricultural Science, Zhengzhou University, Zhengzhou 450001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Agricultural Science, Zhengzhou University, Zhengzhou 450001</wicri:regionArea><wicri:noRegion>Zhengzhou 450001</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Xiwu" sort="Gao, Xiwu" uniqKey="Gao X" first="Xiwu" last="Gao">Xiwu Gao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Entomology, China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Entomology, China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liang, Pei" sort="Liang, Pei" uniqKey="Liang P" first="Pei" last="Liang">Pei Liang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Entomology, China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Entomology, China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Insects</title><idno type="ISSN">2075-4450</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants accumulate phenolic compounds such as tannic acid to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of glutathione S-transferases (GSTs). <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) is an important foliar pest of poplar trees. GSTs play an important role in xenobiotic detoxification in <i>M. troglodyta</i>. Five GST genes were identified in <i>M. troglodyta</i> and were classified into five different cytosolic GST classes, delta, omega, sigma, theta, and zeta. Real-time fluorescent quantitative polymerase chain reaction (qPCR) was used to determine the mRNA expression of the five cloned GSTs in the midguts and fat bodies of <i>M. troglodyta</i>. The mRNA expression of the five GSTs was significantly induced when <i>M. troglodyta</i> was exposed to tannic acid. To further understand the tannic acid regulatory cascade, the 5'-flanking promoter sequences of the five <i>MtGST</i>s were isolated by genome walking methods, and the promoters were very active and induced by tannic acid. In summary, the induction of GST mRNA expression was due to the response of five <i>MtGST</i> promoters to tannic acid. Therefore, <i>MtGST</i> promoters play an important role in the regulation of GST transcription.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32492871</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2075-4450</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>Jun</Month><Day>01</Day></PubDate></JournalIssue><Title>Insects</Title><ISOAbbreviation>Insects</ISOAbbreviation></Journal><ArticleTitle>Molecular Cloning and Characterization of Five Glutathione S-Transferase Genes and Promoters from <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) and Their Response to Tannic Acid Stress.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E339</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/insects11060339</ELocationID><Abstract><AbstractText>Plants accumulate phenolic compounds such as tannic acid to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of glutathione S-transferases (GSTs). <i>Micromelalopha troglodyta</i> (Graeser) (Lepidoptera: Notodontidae) is an important foliar pest of poplar trees. GSTs play an important role in xenobiotic detoxification in <i>M. troglodyta</i>. Five GST genes were identified in <i>M. troglodyta</i> and were classified into five different cytosolic GST classes, delta, omega, sigma, theta, and zeta. Real-time fluorescent quantitative polymerase chain reaction (qPCR) was used to determine the mRNA expression of the five cloned GSTs in the midguts and fat bodies of <i>M. troglodyta</i>. The mRNA expression of the five GSTs was significantly induced when <i>M. troglodyta</i> was exposed to tannic acid. To further understand the tannic acid regulatory cascade, the 5'-flanking promoter sequences of the five <i>MtGST</i>s were isolated by genome walking methods, and the promoters were very active and induced by tannic acid. In summary, the induction of GST mRNA expression was due to the response of five <i>MtGST</i> promoters to tannic acid. Therefore, <i>MtGST</i> promoters play an important role in the regulation of GST transcription.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Fang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Forestry, Nanjing Forestry University, Nanjing 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tu</LastName><ForeName>Huizhen</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Forestry, Nanjing Forestry University, Nanjing 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shang</LastName><ForeName>Qingli</ForeName><Initials>Q</Initials><Identifier Source="ORCID">0000-0002-9631-9838</Identifier><AffiliationInfo><Affiliation>School of Agricultural Science, Zhengzhou University, Zhengzhou 450001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Xiwu</ForeName><Initials>X</Initials><Identifier Source="ORCID">0000-0003-3854-2449</Identifier><AffiliationInfo><Affiliation>Department of Entomology, China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Pei</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Entomology, China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>31370652, 30600476 and 30972376)</GrantID><Agency>the National Natural Science Foundation of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Insects</MedlineTA><NlmUniqueID>101574235</NlmUniqueID><ISSNLinking>2075-4450</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Micromelalopha troglodyta</Keyword><Keyword MajorTopicYN="N">glutathione S-transferases</Keyword><Keyword MajorTopicYN="N">promoter</Keyword><Keyword MajorTopicYN="N">tannic acid</Keyword><Keyword MajorTopicYN="N">transcriptional regulation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>05</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>5</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32492871</ArticleId><ArticleId IdType="pii">insects11060339</ArticleId><ArticleId IdType="doi">10.3390/insects11060339</ArticleId><ArticleId IdType="pmc">PMC7349759</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Drug Resist Updat. 1999 Jun;2(3):153-164</Citation><ArticleIdList><ArticleId 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last="Gao">Xiwu Gao</name><name sortKey="Liang, Pei" sort="Liang, Pei" uniqKey="Liang P" first="Pei" last="Liang">Pei Liang</name><name sortKey="Shang, Qingli" sort="Shang, Qingli" uniqKey="Shang Q" first="Qingli" last="Shang">Qingli Shang</name><name sortKey="Tang, Fang" sort="Tang, Fang" uniqKey="Tang F" first="Fang" last="Tang">Fang Tang</name><name sortKey="Tu, Huizhen" sort="Tu, Huizhen" uniqKey="Tu H" first="Huizhen" last="Tu">Huizhen Tu</name><name sortKey="Tu, Huizhen" sort="Tu, Huizhen" uniqKey="Tu H" first="Huizhen" last="Tu">Huizhen Tu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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